姓名  方谱县

职称：教授

邮箱：Fangpx@sdau.edu.cn; pxfang1990@163.com

办公室：N312

个人主页：https://www.researchgate.net/profile/Puxian-Fang/research

个人简介

方谱县，男，博士，教授，博士生导师，泰山学者青年专家。国家自然科学基金委评审专家、全国研究生教育评估监测专家库专家、中国畜牧兽医学会高级会员。主要从事猪δ冠状病毒、猪流行性腹泻病毒等引起仔猪腹泻的重要动物病毒致病与免疫机制及防控技术研究。先后主持了国家自然科学基金面上项目、青年科学基金、博士后特别资助与面上一等资助各1项，山东省重点研发计划1项。作为子课题负责人参与科技部“十四五”国家重点研发计划项目2项。以第一作者和通讯作者（含共同）在Nucleic Acids Research、Emerging Microbes & Infections、Journal of Virology等杂志上发表SCI论文20余篇，获湖北省科技进步一等奖1项。

教育经历

2015 - 2018 , 农学博士, 预防兽医学, 华中农业大学
2012 - 2015 , 农学硕士, 预防兽医学, 南京农业大学
2008 - 2012 , 农学学士, 动物医学, 江西农业大学

工作经历

2024.05-至今, 教授, 山东农业大学动物医学院

2018.07-2024.04, 博士后, 华中农业大学动物科技学院动物医学院

荣誉奖励

泰山学者青年专家，2024

湖北省科技进步一等奖（第14位），2022年

中国畜牧兽医学会动物传染病分会第十届全体会员代表大会暨第二十次全国学术研讨会优秀报告奖，2023年

执教课程

兽医流行病学、预防兽医学专题等课程

研究领域

1. 动物病毒与宿主相互作用研究

2. 动物腹泻病的病原学及发病机制研究

3. 猪腹泻病防控技术研究

承担的科研项目

1、国家自然科学基金面上项目（32272983），猪δ冠状病毒辅助蛋白NS7a诱导细胞膜融合的机制及其生物学意义，2023-2026

2、国家自然科学基金青年科学基金（31902247），猪δ冠状病毒辅助蛋白NS6调控内质网应激的分子机制研究，2020-2022

3、博士后科学基金特别资助（2019T120670），猪δ冠状病毒调控未折叠蛋白反应的分子机制研究，2019-2023

4、博士后科学基金面上资助（2018M640717），猪δ冠状病毒辅助蛋白NS7a拮抗IFN-β产生的机制研究，2018-2023

5、“十四五”国家重点研发计划，“畜禽冠状病毒的遗传变异、致病与免疫机制”项目子课题，2021-2024

6、“十四五”国家重点研发计划，“猪病毒性疫病新型疫苗创制”项目子课题， 2022-2025

7、泰山学者青年专家项目（tsqn202408118），抗猪δ冠状病毒的小肠黏液蛋白挖掘及作用机制研究，2025.01-2027.12

8、山东省重点研发计划（2025CXPT153），猪重要人兽共患病病原的致病机制及防控产品研究，2025-07-2028-07

发表文章、专利

（一）文章

1.Fang P^†, Xiao W^†, Wang X^†, Xiong Y, Ding T, Cheng T, Chen C, Xiao S^*, Fang L^*.Identification of TMEM41B TM6 as a critical domain for coronavirus replication in vitro and in vivo. Journal of Virology, 2026, 100(8):e0049326.

2.Fang P^†，Xie C^†, Cheng T, Sui J, Cheng Y, Ding T, Guo J, Zhang Y, Fang L, Wei D^*, Xiao S^*. Optimal stability of a highly conserved RNA G4 in PDCoV nsp8 supports viral proliferation. RNA, 2026, 32(5):672-687.

3.Xiao W, Chen C, Xia S, Li Z, Ding T, Zhou J, Fang L, Fang P^*, and Xiao S^*. Cell-surface D-glucuronyl C5-epimerase binds to porcine deltacoronavirus spike protein facilitating viral entry. Journal of Virology, 2024, 98(8):e0088024.

4.Li Z, Lai Y, Qiu R, Tang W, Ren J, Xiao S, Fang P^*, Fang L^*. Hyperacetylated microtubules assist porcine deltacoronavirus nsp8 to degrade MDA5 via SQSTM1/p62-dependent selective autophagy.Journal of Virology, 2024, 98(3): e0000324.

5.Li Z, Xiao W, Yang Z, Guo J, Zhou J, Xiao S, Fang P^* and Fang L^*. Cleavage of HDAC6 to dampen its antiviral activity by nsp5 is a common strategy of swine enteric coronaviruses. Journal of Virology, 2024, 98(2):e0181423.

6.Fang P^†, Xie C^†, Pan T^†, Chen T, Chen W, Xia S, Ding T, Fang J, Zhou Y, Fang L, Wei D^*, and Xiao S^*. Unfolding of an RNA G-quadruplex motif in the negative strand genome of PRRSV by host and viral helicases to promote viral replication. Nucleic Acids Research, 2023, 51(19):10752-10767.

7.Xia S, Xiao W, Zhu X, Liao S, Guo J, Zhou J, Xiao S, Fang P^*, and Fang L^*. Porcine deltacoronavirus resists evades neutralizing antibody neutralization through cell-to-cell transmission. Emerging Microbes & Infections, 2023, 12(1):2207688.

8.Fang P, Zhang H, Cheng T, Ding T, Xia S, Xiao W, Li Z, Xiao S^*, and Fang L^*. Porcine deltacoronavirus accessory protein NS6 harnesses VPS35-mediated retrograde trafficking to facilitate efficient viral infection. Journal of Virology, 2023, 97(10): e0095723.

9.Li Z, Duan P, Qiu R, Fang L, Fang P^*, and Xiao S^*. HDAC6 degrades nsp8 of porcine deltacoronavirus through deacetylation and ubiquitination to inhibit viral replication. Journal of Virology, 2023, 97(5):e0037523.

10.Zhang J, Fang P^*, Ren J, Xia S, Zhang H, Zhu X, Ding T, Xiao S, and Fang L^*. Porcine epidemic diarrhea virus nsp7 inhibits MDA5 dephosphorylation to antagonize type I interferon production. Microbiology Spectrum, 2023, 11(2):e0501722.

11.Li M, Zhang Y, Fang Y, Xiao S, Fang P^*, and Fang L^*. Construction and immunogenicity of a trypsin-independent Porcine epidemic diarrhea virus variant. Frontiers in Immunology,2023, 14:1165606.

12.Ding T, Cheng T, Zhu X, Xiao W, Xia S, Fang L, Fang P^*, and Xiao S^*. Exosomes mediate the antibody-resistant intercellular transmission of porcine epidemic diarrhea virus. Veterinary Microbiology, 2023, 284:109834

13.Fang P, Tian L, Zhang H, Xia S, Ding T, Zhu X, Zhang J, Ren J, Fang L, Xiao S^*. Induction and modulation of the unfolded protein response during porcine deltacoronavirus infection. Veterinary Microbiology, 2022, 271:109494.

14.Ma P, Fang P^*, Ren T, Fang L, Xiao S. Porcine Intestinal Organoids: Overview of the State of the Art. Viruses, 2022, 14(5):1110.

15.Fang P^†, Zhang J^†, Zhang H, Xia S, Ren J, Tian L, Bai D, Fang L, Xiao S^*. Porcine deltacoronavirus enters porcine IPI-2I intestinal epithelial cells via macropinocytosis and clathrin-mediated endocytosis dependent on pH and dynamin.Journal of Virology, 2021, 95(24):e0134521.

16.Fang P, Hong Y, Xia S, Zhang J, Ren J, Zhou Y, Fang L, Xiao S^*. Porcine deltacoronavirus nsp10 antagonizes interferon-β production independently of its zinc finger domains. Virology, 2021, 559:46-56.

17.Fang P, Zhang H, Sun H, Wang G, Xia S, Ren J, Zhang J, Tian L, Fang L, Xiao S^*. Construction, characterization and application of recombinant porcine deltacoronavirus expressing nanoluciferase. Viruses, 2021, 13(10):1991.

18.Fang P, Fang L, Zhang H, Xia S, Xiao S^*. Functions of coronavirus accessory proteins: overview of the state of the art. Viruses, 2021, 13(6):1139.

19.Fang P, Fang L, Xia S, Ren J, Zhang J, Bai D, Zhou Y, Peng G, Zhao S, Xiao S^*. Porcine deltacoronavirus accessory protein NS7a antagonizes IFN-β production by competing with TRAF3 and IRF3 for binding to IKKε. Frontiers in Cellular and Infection Microbiology, 2020, 10:257.

20.Liu S^†, Fang P^†, Ke W, Wang J, Wang X, Xiao S, Fang L^*. Porcine deltacoronavirus (PDCoV) infection antagonizes interferon-λ1 production. Veterinary Microbiology, 2020, 247:108785.

21.Liu X^†, Fang P^†, Fang L, Hong Y, Zhu X, Wang D, Peng G, Xiao S^*. Porcine deltacoronavirus nsp15 antagonizes interferon-β production independently of its endoribonuclease activity. Molecular Immunology, 2019, 114:100-107.

22.Fang P, Fang L, Ren J, Hong Y, Liu X, Zhao Y, Wang D, Peng G, Xiao S^*. Porcine deltacoronavirus accessory protein NS6 antagonizes interferon beta production by interfering with the binding of RIG-I/MDA5 to double-stranded RNA.Journal of Virology, 2018, 92(15):e00712-18.

23.Fang P,Fang L, Hong Y, Liu X, Dong N, Ma P, Bi J, Wang D, Xiao S^*. Discovery of a novel accessory protein NS7a encoded by porcine deltacoronavirus. Journal of General Virology, 2017, 98(2):173-178.

24.Fang P, Fang L, Liu X, Hong Y, Wang Y, Dong N, Ma P, Bi J, Wang D, Xiao S^*. Identification and subcellular localization of porcine deltacoronavirus accessory protein NS6. Virology, 2016, 499:170-177.

25.Fang P, Bai J, Liu X, Dong J, Sun T, Jiang P^*. Construction and characterization of an infectious cDNA clone of encephalomyocarditis virus from pigs in China. Archives of Virology, 2015,160(3):805-809.

26.方谱县, 方六荣, 董楠, 肖少波. 猪δ冠状病毒的研究进展. 病毒学报, 2016, 32(02):243-248.

（二）授权发明专利

1. 方六荣，方谱县，肖少波，夏思进，任杰，张健淞，张慧畅。一种猪δ冠状病毒感染性克隆质粒的构建方法。专利号：ZL202110154305.8。

2. 方六荣，肖少波，方谱县，周艳荣，马俊，卢佑新，彭旋。一株猪δ冠状病毒强毒株及其在疫苗制备中的应用。专利号：ZL202210278623.X。

3. 方六荣，廖树森，肖少波，安楠，潘永光，周艳荣，方谱县。一株针对猪δ冠状病毒S1蛋白构象表位的中和性单克隆抗体。专利号：ZL202211618625.5。

4. 肖少波，方六荣，李明翔，周艳荣，方谱县，卢佑新，彭旋。一种不依赖胰酶的猪流行性腹泻病毒变异毒株及其构建与应用。专利号：ZL202210214959.X。

5. 方六荣，潘永光，肖少波，彭旋，廖树森，周艳荣，方谱县。猪流行性腹泻病毒抗体捕获 ELISA 检测试剂盒。专利号：ZL202311651590X。